Isolation and identification of unknown bacteria Lab Report

Isolation and identification of unknown bacteria - Lab Report Example We used standardized procedures in the process of cultivation of bacetia. We received pure culture of unknown organism. Using Bunsen burner the loop was sterilized by heating the entire wire into the flame of the burner until it glows with bright red or orange color and afterwards the loop was left to cool. The culture of uknown bacteria was held in inoculation tube in a form of liquid culture and the inoulation tube was sealed with sterile cap.. We maintained aseptic conditions during the handling of the culture. During the whole process the caps and the tubes were held in hand were not allowed to be contaminated by contacting the table or other source of environmental bacteria. The openings of the inoculation tube was sterilized by burning with the Bunsen burner twice in order to avoid contamination with environmental bacteria. With the cooled loop, carfully not to touch the sides of the tube we collected one loop of material and after that the edges of the tube were again burned with flame from the Bunsen burner and the caps were put . The loop was then inoculated on two separate TSA plates. The lid of the plates was opened with free hand and the material was seeded with gentle strokes of the loop using T streak method in order to allow optimal contidions for growth. The lid was then put back on the plates and the loop was again sterilized by burning until glowing bright red on the Bunsen burner. ... erilized by heating the entire wire into the flame of the burner until it glows with bright red or orange color and afterwards the loop was left to cool. The culture of uknown bacteria was held in inoculation tube in a form of liquid culture and the inoulation tube was sealed with sterile cap.. We maintained aseptic conditions during the handling of the culture. During the whole process the caps and the tubes were held in hand were not allowed to be contaminated by contacting the table or other source of environmental bacteria. The openings of the inoculation tube was sterilized by burning with the Bunsen burner twice in order to avoid contamination with environmental bacteria. With the cooled loop, carfully not to touch the sides of the tube we collected one loop of material and after that the edges of the tube were again burned with flame from the Bunsen burner and the caps were put . The loop was then inoculated on two separate TSA plates. The lid of the plates was opened with fre e hand and the material was seeded with gentle strokes of the loop using T streak method in order to allow optimal contidions for growth. The lid was then put back on the plates and the loop was again sterilized by burning until glowing bright red on the Bunsen burner. Gram stain For completing the Gram stain we needed Gram stain reagents (crystal violet, Gram's iodine, 95% ethyl alcohol, and safranin), microscope slides and bacterial cultures. In preparation of the Gram stain asepttci principles were used. Inocultion loop was sterilized by burning on a Bunsen burner until bright red color appeared. Five loop of the unknown colony were placed on a microscope slide and the material was sread even on the surface of the slide. The material was left to air-dry and room temperature. After this